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pedv qy 2016 strain  (ATCC)


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    Structured Review

    ATCC pedv qy 2016 strain
    Preparation and characterization of S1-CS nanoparticles . A Effect of the pH of the chitosan solution on the solubility of the <t>PEDV</t> S1 protein. B Effects of the ratio of chitosan to S1 protein on the encapsulation efficiency (EE) and loading efficiency (LE) of S1-CS NPs. The dotted line represents the precipitation. C SDS‒PAGE analysis of the antigen content in S1-CS NPs. D TEM image of S1-CS NPs at 200 000 × magnification. E Cell viability evaluation of S1-CS NPs. As determined by the CCK-8 assay after 24 h. F Stability analysis of S1-CS in the simulated gastric fluid. CS, free S1 protein and S1-CS NPs were incubated with simulated gastric fluid (pH 1.2). All the data are presented as the mean ± SD.
    Pedv Qy 2016 Strain, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 981 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pedv+qy+2016+strain/Sp2%2FmIL-6/pmc12829007-45-14-11
    Average 96 stars, based on 981 article reviews
    pedv qy 2016 strain - by Bioz Stars, 2026-09
    96/100 stars

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    1) Product Images from "Intestinal mucosal immune responses induced by oral administration of chitosan nanoparticles encapsulating the PEDV S1 protein"

    Article Title: Intestinal mucosal immune responses induced by oral administration of chitosan nanoparticles encapsulating the PEDV S1 protein

    Journal: Veterinary Research

    doi: 10.1186/s13567-025-01695-6

    Preparation and characterization of S1-CS nanoparticles . A Effect of the pH of the chitosan solution on the solubility of the PEDV S1 protein. B Effects of the ratio of chitosan to S1 protein on the encapsulation efficiency (EE) and loading efficiency (LE) of S1-CS NPs. The dotted line represents the precipitation. C SDS‒PAGE analysis of the antigen content in S1-CS NPs. D TEM image of S1-CS NPs at 200 000 × magnification. E Cell viability evaluation of S1-CS NPs. As determined by the CCK-8 assay after 24 h. F Stability analysis of S1-CS in the simulated gastric fluid. CS, free S1 protein and S1-CS NPs were incubated with simulated gastric fluid (pH 1.2). All the data are presented as the mean ± SD.
    Figure Legend Snippet: Preparation and characterization of S1-CS nanoparticles . A Effect of the pH of the chitosan solution on the solubility of the PEDV S1 protein. B Effects of the ratio of chitosan to S1 protein on the encapsulation efficiency (EE) and loading efficiency (LE) of S1-CS NPs. The dotted line represents the precipitation. C SDS‒PAGE analysis of the antigen content in S1-CS NPs. D TEM image of S1-CS NPs at 200 000 × magnification. E Cell viability evaluation of S1-CS NPs. As determined by the CCK-8 assay after 24 h. F Stability analysis of S1-CS in the simulated gastric fluid. CS, free S1 protein and S1-CS NPs were incubated with simulated gastric fluid (pH 1.2). All the data are presented as the mean ± SD.

    Techniques Used: Solubility, Encapsulation, CCK-8 Assay, Incubation

    Related Articles

    Solubility:

    Article Title: Intestinal mucosal immune responses induced by oral administration of chitosan nanoparticles encapsulating the PEDV S1 protein
    Article Snippet: The African green monkey kidney epithelial (Vero)-CCL81 cell line from the ATCC and the PEDV QY-2016 strain (GenBank ID: MH244927) were preserved in our laboratory.. The Vero-CCL81 cells were propagated in Dulbecco’s modified Eagle’s medium (DMEM; Gibco, California, USA) supplemented with 10% FBS (Sigma Aldrich, USA), 100 IU/mL penicillin, and 100 μg/mL streptomycin (Solarbio, Beijing, China) in a humidified incubator at 37 °C and 5% CO 2 .The Vero-CCL81 cells were propagated in Dulbecco’s modified Eagle’s medium (DMEM; Gibco, California, USA) supplemented with 10% FBS (Sigma Aldrich, USA), 100 IU/mL penicillin, and 100 μg/mL streptomycin (Solarbio, Beijing, China) in a humidified incubator at 37 °C and 5% CO 2 .

    Article Title: Intestinal mucosal immune responses induced by oral administration of chitosan nanoparticles encapsulating the PEDV S1 protein.
    Article Snippet: The African green monkey kidney epithelial (Vero)-CCL81 cell line from the ATCC and the PEDV QY-2016 strain (GenBank ID: MH244927) were preserved in our laboratory.. The VeroCCL81 cells were propagated in Dulbecco’s modified Eagle’s medium (DMEM; Gibco, California, USA) supplemented with 10% FBS (Sigma Aldrich, USA), 100 IU/mL penicillin, and 100 μg/mL streptomycin (Solarbio, Beijing, China) in a humidified incubator at 37 °C and 5% CO2.The VeroCCL81 cells were propagated in Dulbecco’s modified Eagle’s medium (DMEM; Gibco, California, USA) supplemented with 10% FBS (Sigma Aldrich, USA), 100 IU/mL penicillin, and 100 μg/mL streptomycin (Solarbio, Beijing, China) in a humidified incubator at 37 °C and 5% CO2.

    Encapsulation:

    Article Title: Intestinal mucosal immune responses induced by oral administration of chitosan nanoparticles encapsulating the PEDV S1 protein
    Article Snippet: The African green monkey kidney epithelial (Vero)-CCL81 cell line from the ATCC and the PEDV QY-2016 strain (GenBank ID: MH244927) were preserved in our laboratory.. The Vero-CCL81 cells were propagated in Dulbecco’s modified Eagle’s medium (DMEM; Gibco, California, USA) supplemented with 10% FBS (Sigma Aldrich, USA), 100 IU/mL penicillin, and 100 μg/mL streptomycin (Solarbio, Beijing, China) in a humidified incubator at 37 °C and 5% CO 2 .The Vero-CCL81 cells were propagated in Dulbecco’s modified Eagle’s medium (DMEM; Gibco, California, USA) supplemented with 10% FBS (Sigma Aldrich, USA), 100 IU/mL penicillin, and 100 μg/mL streptomycin (Solarbio, Beijing, China) in a humidified incubator at 37 °C and 5% CO 2 .

    Article Title: Intestinal mucosal immune responses induced by oral administration of chitosan nanoparticles encapsulating the PEDV S1 protein.
    Article Snippet: The African green monkey kidney epithelial (Vero)-CCL81 cell line from the ATCC and the PEDV QY-2016 strain (GenBank ID: MH244927) were preserved in our laboratory.. The VeroCCL81 cells were propagated in Dulbecco’s modified Eagle’s medium (DMEM; Gibco, California, USA) supplemented with 10% FBS (Sigma Aldrich, USA), 100 IU/mL penicillin, and 100 μg/mL streptomycin (Solarbio, Beijing, China) in a humidified incubator at 37 °C and 5% CO2.The VeroCCL81 cells were propagated in Dulbecco’s modified Eagle’s medium (DMEM; Gibco, California, USA) supplemented with 10% FBS (Sigma Aldrich, USA), 100 IU/mL penicillin, and 100 μg/mL streptomycin (Solarbio, Beijing, China) in a humidified incubator at 37 °C and 5% CO2.

    CCK-8 Assay:

    Article Title: Intestinal mucosal immune responses induced by oral administration of chitosan nanoparticles encapsulating the PEDV S1 protein
    Article Snippet: The African green monkey kidney epithelial (Vero)-CCL81 cell line from the ATCC and the PEDV QY-2016 strain (GenBank ID: MH244927) were preserved in our laboratory.. The Vero-CCL81 cells were propagated in Dulbecco’s modified Eagle’s medium (DMEM; Gibco, California, USA) supplemented with 10% FBS (Sigma Aldrich, USA), 100 IU/mL penicillin, and 100 μg/mL streptomycin (Solarbio, Beijing, China) in a humidified incubator at 37 °C and 5% CO 2 .The Vero-CCL81 cells were propagated in Dulbecco’s modified Eagle’s medium (DMEM; Gibco, California, USA) supplemented with 10% FBS (Sigma Aldrich, USA), 100 IU/mL penicillin, and 100 μg/mL streptomycin (Solarbio, Beijing, China) in a humidified incubator at 37 °C and 5% CO 2 .

    Article Title: Intestinal mucosal immune responses induced by oral administration of chitosan nanoparticles encapsulating the PEDV S1 protein.
    Article Snippet: The African green monkey kidney epithelial (Vero)-CCL81 cell line from the ATCC and the PEDV QY-2016 strain (GenBank ID: MH244927) were preserved in our laboratory.. The VeroCCL81 cells were propagated in Dulbecco’s modified Eagle’s medium (DMEM; Gibco, California, USA) supplemented with 10% FBS (Sigma Aldrich, USA), 100 IU/mL penicillin, and 100 μg/mL streptomycin (Solarbio, Beijing, China) in a humidified incubator at 37 °C and 5% CO2.The VeroCCL81 cells were propagated in Dulbecco’s modified Eagle’s medium (DMEM; Gibco, California, USA) supplemented with 10% FBS (Sigma Aldrich, USA), 100 IU/mL penicillin, and 100 μg/mL streptomycin (Solarbio, Beijing, China) in a humidified incubator at 37 °C and 5% CO2.

    Incubation:

    Article Title: Intestinal mucosal immune responses induced by oral administration of chitosan nanoparticles encapsulating the PEDV S1 protein
    Article Snippet: The African green monkey kidney epithelial (Vero)-CCL81 cell line from the ATCC and the PEDV QY-2016 strain (GenBank ID: MH244927) were preserved in our laboratory.. The Vero-CCL81 cells were propagated in Dulbecco’s modified Eagle’s medium (DMEM; Gibco, California, USA) supplemented with 10% FBS (Sigma Aldrich, USA), 100 IU/mL penicillin, and 100 μg/mL streptomycin (Solarbio, Beijing, China) in a humidified incubator at 37 °C and 5% CO 2 .The Vero-CCL81 cells were propagated in Dulbecco’s modified Eagle’s medium (DMEM; Gibco, California, USA) supplemented with 10% FBS (Sigma Aldrich, USA), 100 IU/mL penicillin, and 100 μg/mL streptomycin (Solarbio, Beijing, China) in a humidified incubator at 37 °C and 5% CO 2 .

    Article Title: Intestinal mucosal immune responses induced by oral administration of chitosan nanoparticles encapsulating the PEDV S1 protein.
    Article Snippet: The African green monkey kidney epithelial (Vero)-CCL81 cell line from the ATCC and the PEDV QY-2016 strain (GenBank ID: MH244927) were preserved in our laboratory.. The VeroCCL81 cells were propagated in Dulbecco’s modified Eagle’s medium (DMEM; Gibco, California, USA) supplemented with 10% FBS (Sigma Aldrich, USA), 100 IU/mL penicillin, and 100 μg/mL streptomycin (Solarbio, Beijing, China) in a humidified incubator at 37 °C and 5% CO2.The VeroCCL81 cells were propagated in Dulbecco’s modified Eagle’s medium (DMEM; Gibco, California, USA) supplemented with 10% FBS (Sigma Aldrich, USA), 100 IU/mL penicillin, and 100 μg/mL streptomycin (Solarbio, Beijing, China) in a humidified incubator at 37 °C and 5% CO2.

    Cell Culture:

    Article Title: Intestinal mucosal immune responses induced by oral administration of chitosan nanoparticles encapsulating the PEDV S1 protein
    Article Snippet: The African green monkey kidney epithelial (Vero)-CCL81 cell line from the ATCC and the PEDV QY-2016 strain (GenBank ID: MH244927) were preserved in our laboratory.. The Vero-CCL81 cells were propagated in Dulbecco’s modified Eagle’s medium (DMEM; Gibco, California, USA) supplemented with 10% FBS (Sigma Aldrich, USA), 100 IU/mL penicillin, and 100 μg/mL streptomycin (Solarbio, Beijing, China) in a humidified incubator at 37 °C and 5% CO 2 .The Vero-CCL81 cells were propagated in Dulbecco’s modified Eagle’s medium (DMEM; Gibco, California, USA) supplemented with 10% FBS (Sigma Aldrich, USA), 100 IU/mL penicillin, and 100 μg/mL streptomycin (Solarbio, Beijing, China) in a humidified incubator at 37 °C and 5% CO 2 .

    Article Title: Intestinal mucosal immune responses induced by oral administration of chitosan nanoparticles encapsulating the PEDV S1 protein.
    Article Snippet: The African green monkey kidney epithelial (Vero)-CCL81 cell line from the ATCC and the PEDV QY-2016 strain (GenBank ID: MH244927) were preserved in our laboratory.. The VeroCCL81 cells were propagated in Dulbecco’s modified Eagle’s medium (DMEM; Gibco, California, USA) supplemented with 10% FBS (Sigma Aldrich, USA), 100 IU/mL penicillin, and 100 μg/mL streptomycin (Solarbio, Beijing, China) in a humidified incubator at 37 °C and 5% CO2.The VeroCCL81 cells were propagated in Dulbecco’s modified Eagle’s medium (DMEM; Gibco, California, USA) supplemented with 10% FBS (Sigma Aldrich, USA), 100 IU/mL penicillin, and 100 μg/mL streptomycin (Solarbio, Beijing, China) in a humidified incubator at 37 °C and 5% CO2.



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    ATCC pedv qy 2016 strain
    Preparation and characterization of S1-CS nanoparticles . A Effect of the pH of the chitosan solution on the solubility of the <t>PEDV</t> S1 protein. B Effects of the ratio of chitosan to S1 protein on the encapsulation efficiency (EE) and loading efficiency (LE) of S1-CS NPs. The dotted line represents the precipitation. C SDS‒PAGE analysis of the antigen content in S1-CS NPs. D TEM image of S1-CS NPs at 200 000 × magnification. E Cell viability evaluation of S1-CS NPs. As determined by the CCK-8 assay after 24 h. F Stability analysis of S1-CS in the simulated gastric fluid. CS, free S1 protein and S1-CS NPs were incubated with simulated gastric fluid (pH 1.2). All the data are presented as the mean ± SD.
    Pedv Qy 2016 Strain, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pedv+qy+2016+strain/Sp2%2FmIL-6/pmc12829007-45-14-11
    Average 96 stars, based on 1 article reviews
    pedv qy 2016 strain - by Bioz Stars, 2026-09
    96/100 stars
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    Preparation and characterization of S1-CS nanoparticles . A Effect of the pH of the chitosan solution on the solubility of the PEDV S1 protein. B Effects of the ratio of chitosan to S1 protein on the encapsulation efficiency (EE) and loading efficiency (LE) of S1-CS NPs. The dotted line represents the precipitation. C SDS‒PAGE analysis of the antigen content in S1-CS NPs. D TEM image of S1-CS NPs at 200 000 × magnification. E Cell viability evaluation of S1-CS NPs. As determined by the CCK-8 assay after 24 h. F Stability analysis of S1-CS in the simulated gastric fluid. CS, free S1 protein and S1-CS NPs were incubated with simulated gastric fluid (pH 1.2). All the data are presented as the mean ± SD.

    Journal: Veterinary Research

    Article Title: Intestinal mucosal immune responses induced by oral administration of chitosan nanoparticles encapsulating the PEDV S1 protein

    doi: 10.1186/s13567-025-01695-6

    Figure Lengend Snippet: Preparation and characterization of S1-CS nanoparticles . A Effect of the pH of the chitosan solution on the solubility of the PEDV S1 protein. B Effects of the ratio of chitosan to S1 protein on the encapsulation efficiency (EE) and loading efficiency (LE) of S1-CS NPs. The dotted line represents the precipitation. C SDS‒PAGE analysis of the antigen content in S1-CS NPs. D TEM image of S1-CS NPs at 200 000 × magnification. E Cell viability evaluation of S1-CS NPs. As determined by the CCK-8 assay after 24 h. F Stability analysis of S1-CS in the simulated gastric fluid. CS, free S1 protein and S1-CS NPs were incubated with simulated gastric fluid (pH 1.2). All the data are presented as the mean ± SD.

    Article Snippet: The African green monkey kidney epithelial (Vero)-CCL81 cell line from the ATCC and the PEDV QY-2016 strain (GenBank ID: MH244927) were preserved in our laboratory.

    Techniques: Solubility, Encapsulation, CCK-8 Assay, Incubation